explorer antibody microarrays asb600 (Full Moon BioSystems)
Structured Review

Explorer Antibody Microarrays Asb600, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/explorer+antibody+microarrays+asb600/explorer+antibody+microarrays+asb600/pmc11708116-169-3-7
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "P-, E-, and H-cadherins differ in their relationships with coronary stenosis, cardiovascular outcomes, and unplanned recurrent revascularization"
Article Title: P-, E-, and H-cadherins differ in their relationships with coronary stenosis, cardiovascular outcomes, and unplanned recurrent revascularization
Journal: Journal of Molecular and Cellular Cardiology Plus
doi: 10.1016/j.jmccpl.2024.100091
Figure Legend Snippet: Image of the Explorer antibody microarrays (ASB600, Full Moon Biosystems, USA) with 656 antibodies per slide in two replicates for each antibody (left). Labeled load of serum proteins was 500 ng; scan settings: velocity: 20 l/s; laser power: 5.0; detector gain: 100; pixel size: 10; wavelength: 532 nm. Enlarged images (on right) illustrate the examples of the differences in the levels of cadherins (Pan), E -cadherin, and P-cadherin in the serum of patient (total N = 18) with and without coronary lesions ( N = 9) (A) and with coronary stenosis (N = 9) (B). Differences in the levels of cadherins were analyzed using Mann-Whitney U test. Quantification of the images is shown in to indicate that the P values <0.05 were detected for E- and P-cadherins. Each sample was analyzed in duplicate.
Techniques Used: Labeling, MANN-WHITNEY
Related Articles
Labeling:Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins Article Snippet: .. Labeled proteins (500 ng per slide in blocking buffer containing 3 % dry milk) were processed using Blocking Assay:Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins Article Snippet: .. Labeled proteins (500 ng per slide in blocking buffer containing 3 % dry milk) were processed using Microarray:Article Title: Atrial fibrillation recurrence after catheter ablation is associated with RAD51 and p63 proteins Article Snippet: .. Labeled proteins (500 ng per slide in blocking buffer containing 3 % dry milk) were processed using Incubation:Article Title: Associations of adenovirus-reactive immunoglobulins with atrial fibrillation and body mass index Article Snippet: The reaction was terminated by the addition of 10 μl of stop solution (Arrayit Corp.). .. After 30 min incubation on ice, inactivated unbound dye was removed by gel filtration through spin columns by centrifugation at 750 g for 2 min. Article Title: Application of an antibody microarray for serum protein profiling of coronary artery stenosis. Article Snippet: Protein expression profiling in the serum is used to identify novel biomarkers and investigate the signaling pathways in various diseases.. The aim of the present study was to evaluate serum biomarkers associated with coronary artery stenosis resulting from atherosclerosis.. The study included 4 groups of subjects: group A and B with and without coronary lesions, respectively, were selected from a previously reported cohort study on coronary atherosclerosis, control group C comprised of asymptomatic subjects and group D was used for independent validation of the microarray data by ELISA. Filtration:Article Title: Associations of adenovirus-reactive immunoglobulins with atrial fibrillation and body mass index Article Snippet: The reaction was terminated by the addition of 10 μl of stop solution (Arrayit Corp.). .. After 30 min incubation on ice, inactivated unbound dye was removed by gel filtration through spin columns by centrifugation at 750 g for 2 min. Article Title: Application of an antibody microarray for serum protein profiling of coronary artery stenosis. Article Snippet: Protein expression profiling in the serum is used to identify novel biomarkers and investigate the signaling pathways in various diseases.. The aim of the present study was to evaluate serum biomarkers associated with coronary artery stenosis resulting from atherosclerosis.. The study included 4 groups of subjects: group A and B with and without coronary lesions, respectively, were selected from a previously reported cohort study on coronary atherosclerosis, control group C comprised of asymptomatic subjects and group D was used for independent validation of the microarray data by ELISA. Centrifugation:Article Title: Associations of adenovirus-reactive immunoglobulins with atrial fibrillation and body mass index Article Snippet: The reaction was terminated by the addition of 10 μl of stop solution (Arrayit Corp.). .. After 30 min incubation on ice, inactivated unbound dye was removed by gel filtration through spin columns by centrifugation at 750 g for 2 min. Article Title: Application of an antibody microarray for serum protein profiling of coronary artery stenosis. Article Snippet: Protein expression profiling in the serum is used to identify novel biomarkers and investigate the signaling pathways in various diseases.. The aim of the present study was to evaluate serum biomarkers associated with coronary artery stenosis resulting from atherosclerosis.. The study included 4 groups of subjects: group A and B with and without coronary lesions, respectively, were selected from a previously reported cohort study on coronary atherosclerosis, control group C comprised of asymptomatic subjects and group D was used for independent validation of the microarray data by ELISA. |
